Bovine Collagen Solution, Type I, 3 mg/ml, 1000 ml PureCol®, Bovine Collagen, 3 mg/mL, 1000 mL 货号：BM-5005-1000ML 品牌：Advanced Biomatrix -Equl意果_易扩_AdvancedBioMatrix_DivBio_Drummond_Genie_Glascol_Megazyme_Phadebas_Worthington

来自 : 发布时间：2024-12-23

Bovine Collagen Solution, Type I, 3 mg/ml, 1000 ml PureCol®, Bovine Collagen, 3 mg/mL, 1000 mL 货号：BM-5005-1000ML 品牌：Advanced BioMatrix -Equl意果_易扩_AdvancedBioMatrix_DivBio_Drummond_Genie_Glascol_Megazyme_Phadebas_Worthington

英文名：PureCol®, Bovine Collagen, 3 mg/mL, 1000 mL

货号：BM-5005-1000ML

规格：1000ML

品牌： Advanced BioMatrix

PureCol collagen is known as the standard of all collagens for purity ( 99.9% collagen content), functionality, and the most native-like collagen available. PureCol is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

PureCol collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. PureCol collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. PureCol collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.

PureCol collagen is provided in a user-friendly packaging for use and storage. PureCol is sterile filtered and is supplied as a ready to use solution.

Purecol胶原蛋白被称为标准纯度的胶原蛋白（ 99.9%胶原蛋白含量），其功能可媲美大多数的原生胶原蛋白。Purecol提取于来自美国封闭式牛群的牛皮，先进的生物生产过程符合严格的质量标准，已被证明是质量超凡的。Purecol胶原蛋白是由约97%的I型胶原蛋白，其余的为III型胶原蛋白组成的，它具有很高的单体含量可以用于测试凝胶渗透色谱。本产品的浓度约为3mg/ml，每个批次的具体浓度会写于产品的COA上随货发出。PureCol是溶于0.01N盐酸的端胶原蛋白，因此它的PH值约为2。PureCol胶原蛋白是理想的蛋白质涂层，可用于准备培养的细胞或者固体凝胶。PureCol胶原蛋白的包装便于储存和使用，经过灭菌的溶液随时可以使用。

Note:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

Remove required quantity of collagen from the bottle and dispense into a dilution vessel. Dilute PureCol in water to ~50 to 100 g/ml (~1:30). A 0.01 M HCl solution may also be used. Swirl contents gently until material is completely mixed. Add appropriate amount of diluted PureCol material to the culture surface ensuring that the entire surface is coated. Incubate at room temperature or 37 C, covered, for 1-2 hours. After incubation, aspirate any remaining material. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces. Coated surfaces are ready for use. They may also be stored at 2-8 C damp or air dried if sterility is maintained.

3-D Gel Preparation Procedure

Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling. Adjust pH of mixture to 7.2 7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper). Adjust final volume to a total of 10 parts with sterile water. To prevent gelation, maintain temperature of mixture at 2 10 C. To form gel, warm to 37 C. Allow approximately 90 to 120 minutes for gel formation.

Cell Attachment Bioassay

To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37 C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.

注意事项：

This product is for R D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

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