ProductDescription
Poly-L-Ornithineisasyntheticaminoacidchainthatispositivelychargedandwidelyusedasacoatingtoenhancecellattachmentandadhesiontobothplasticwareandglasssurfaces.
ThemolecularweightofPoly-L-Ornithinecanvarysignificantlywithlowermolecularweight(30,000Da)beinglessviscousandhighermolecularweight(>300,000Da)havingmorebindingsitespermolecule.Thisproduct’smolecularweightrangesfrom70,000to150,000Da.
Poly-L-Ornithineissuppliedina50mlamberbottlecontaining5mgofPoly-L-Ornithine.Theproducthasbeensterilizedandisready-to-useafterproperadditionofcellculturewater.
| Parameter,Testing,andMethod | Poly-L-Ornithine#5172 |
| Form | Powder |
| PackageSize | 5mg |
| StorageTemperature | -20°C |
| SterilizationMethod | IrrADIation |
| MolecularWeight | 70,000-150,000Da |
| Sterility-USPmodified | NoGrowth |
| CellAttachmentAssay | Pass |
| Source | Synthetic |
| ShelfLife | Minimumof6monthsfromdateofreceipt |
DirectionsforUse
DownloadthefullPDFversionorcontinuereadingbelow:
Usetheserecommendationsasguidelinestodeterminetheoptimalcoatingconditionsforyourculturesystem.Tomaintainsterility,performalloperationsinalaminarflowhood.
- Atypicalworkingconcentrationis0.1mg/ml.Toachievea0.1mg/mlconcentration,add50mloftissueculturegradewaterorPBStothe50mlbottle.Ifadifferentconcentrationisdesired,transferdesiredvolumeofsolutionfromthebottletoadilutionvessel.DilutetodesiredconcentrationusingtissueculturegradewaterorPBS.
- Addappropriateamountofdilutedmaterialtoculturesurface.Typically,1mlper25cm2isused.Rockgentlytoensureuniformcoatingofculturesurface.
OptionA:
- Allowtoincubatefor60minutesatroomtemperature,removeexcesssolutionbyaspiration.
- Thoroughlyrinsesurfacewithtissueculturegradewaterandaspiraterinsewater.
- Incubateandallowtodryatroomtemperatureor37°C,covered,foratleast2hours.Coatedculturewarecanbestoredforupto1week.GotoStep9
OptionB:
- Allowtoincubateovernightatroomtemperature,removeexcesssolutionbyaspiration.
- Thoroughlyrinsesurfacewithtissueculturegradewaterandaspiraterinsewater.
- Incubateandallowtodryatroomtemperatureor37°C,covered,foratleast2hours.Coatedculturewarecanbestoredforupto1week.
- Introducemediumandcellstotheculturesurface.
StoreremainingPoly-L-Ornithinesolutionat-10to30°C
ProductReferences
ReferencesforPoly-L-Ornithine:
Chen,EricYT,etal."ActivatedcharcoalcompositeBiomaterialpromoteshumanembryonicstemcelldifferentiationtowardneuronallineage."JournalofBiomedicalMaterialsResearchPartA100.8(2012):2006-2017.
Boularaoui,SelwaMokhtar,etal."Efficienttransdifferentiationofhumandermalfibroblastsintoskeletalmuscle."Journaloftissueengineeringandregenerativemedicine12.2(2018):e918-e936.
Ergin,Ekin,etal."Time-resolvedfluorescenceresonanceenergytransfer[TR-FRET]assaysforbiochemicalprocesses."Currentpharmaceuticalbiotechnology17.14(2016):1222-1230.
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SafetyandDocumentation
SafetyDataSheet
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ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
