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BioMatrix(优势品牌)
主营:胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
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当前位置: 首页 > 产品中心 > Electrophysiological > 高级生物矩阵/聚赖氨酸//5048-50ML
商品详细高级生物矩阵/聚赖氨酸//5048-50ML
高级生物矩阵/聚赖氨酸//5048-50ML
高级生物矩阵/聚赖氨酸//5048-50ML
商品编号: 5048-50ML
品牌: BioMatrix
市场价: ¥1500.00
美元价: 900.00
产地: 美国(厂家直采)
公司:
产品分类: 电生理设备
公司分类: Electrophysiological
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Product Description

Poly-L-Lysineis a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine.Poly-L-Lysineis widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule has been used to culture a wide variety of cell types. Certain cell types secrete proteases, which can digestPoly-L-Lysine. For those cell types, Poly-D-Lysine, Catalog # 5049 should be used.

The molecular weight ofPoly-L-Lysinecan vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-L-Lysinesurface coatings are designed to improve cell attachment, growth and differentiation of many cell types.

Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.

Parameter, Testing, and MethodPoly-L-Lysine #5048
FormSolution
Package Size50 mL
Storage Temperature2-10°C
Sterilization MethodFiltration
Molecular Weight70,000 - 150,000 Da
Sterility - USP modifiedNo Growth
Cell Attachment AssayPass
SourceSynthetic
Shelf LifeMinimum of 6 months from date of receipt
Concentration0.1 mg/mL

Directions for Use

Download the full PDF version or continue reading below:

Use these recommendations as guidelines todetermine the optimal coating conditions for your culture system. To maintain sterility, perform all operations in a laminar flow hood.

A typical working concentration is 0.1 mg/mL. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.

  1. Add appropriate amount of diluted material to culture surface. Typically, 1 ml per 25 cm2is used. Rock gently to ensure uniform coating of culture surface.
  2. After 5 minutes, remove excess solution by aspiration.
  3. Thoroughly rinse surface with tissue culture grade water.
  4. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours.
  5. Introduce medium and cells to the culture surface.
  6. Store remaining Poly-L-Lysine at 2 to 10°C.

Product References

References for Poly-L-Lysine:

Påhlman, Lisa I., et al. "Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-β1."BMC pulmonary medicine15.1 (2015): 19.

Li, Yun, et al. "Imaging pHluorin-tagged receptor insertion to the plasma membrane in primary cultured mouse neurons."JoVE (Journal of Visualized Experiments)69 (2012): e4450.

Morand, David-Nicolas, et al. "Active nanofibrous membrane effects on gingival cell inflammatory response."Materials8.10 (2015): 7217-7229.

Thuault, Sylvie, et al. "The RhoE/ROCK/ARHGAP25 signaling pathway controls cell invasion by inhibition of Rac activity."Molecular biology of the cell27.17 (2016): 2653-2661.

Popielarski, Marcin, et al. "The role of Protein Disulfide Isomerase and thiol bonds modifications in activation of integrin subunit alpha11."Biochemical and biophysical research communications495.2 (2018): 1635-1641.

Wu, Kun Lieh, et al. "Effects of culturing media on hepatocytes differentiation using Volvox sphere as co-culturing vehicle."Biochemical and biophysical research communications458.3 (2015): 620-625.

Santio, Niina M., et al. "The PIM1 kinase promotes prostate cancer cell migration and adhesion via multiple signalling pathways."Experimental cell research342.2 (2016): 113-124.

Product Certificate of Analysis

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Product Videos

link to library blog - Coating a Glass Coverslip with Collagen
Coating a Glass Coverslip with Collagen

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

品牌介绍
美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。 以下为Advanced BioMatrix 3D Matrices 产品竞争优势: 1. 提供高纯度和成分确定的胞外基质; 2. 超过1000余篇文献引用PureCol产品,品质非常均一; 3. 在3D培养基领域可提供最全面的产品线; 4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft); 5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养); 6. 如果客户首次接触3D胶原凝胶,Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。 以下产品为Advanced BioMatrix全球畅销品: 1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML 2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML 3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML 4. VitroCol 人源I型胶原蛋白 #5007-20ML 5. 弹性蛋白原 #5052-1MG 6. ECM Select Array kit Ultra-36 #5170-1EA 7. CytoSoft(刚性可变的基底,Advanced BioMatrix最新添加产品5190-7EA) 8. 人III型胶原蛋白 #5021-10MG 9. 人IV型胶原蛋白 #5022-5MG 10. Silk Fibroin溶液 #5154-20ML 11. Fibronectin #5080-5MG 12. Vitronectin #5051-0.1MG Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。