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BioMatrix(优势品牌)
主营:胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
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当前位置: 首页 > 产品中心 > Electrophysiological > 高级生物基质/ICAM2//5107-0.1MG
商品详细高级生物基质/ICAM2//5107-0.1MG
高级生物基质/ICAM2//5107-0.1MG
高级生物基质/ICAM2//5107-0.1MG
商品编号: 5107-0.1MG
品牌: BioMatrix
市场价: ¥4400.00
美元价: 2640.00
产地: 美国(厂家直采)
公司:
产品分类: 电生理设备
公司分类: Electrophysiological
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Product Description

The protein encoded by human ICAM2 gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene.

Full-length extracellular domain of human ICAM2 gene (25-223 aa) was constructed with 29 N-terminal T7/His tag and expressed in E. coli as inclusion bodies.

The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Parameter, Testing, and MethodICAM2 #5107
Quantity0.1 mg
Volume0.2 mL
Concentration0.5 mg/mL
Purity - SDS PAGE Electrophoresis> 90%
FormulationFormulated in 20 mM pH 8.0 TRIS-HCL Buffer, with proprietary formulation of NaCl, KCl, EDTA, L-Arginine, DTT and Glycerol
FormSolution
Production TypeRecombinant - E. Coli
Storage Temperature-20°C
Shelf LifeMinimum of 6 months from date of receipt
Sterilization MethodFiltration
Cell AssayPass
Sterility- USP modifiedNo Growth
Accession NumberNP_000864
Recombinant Protein SequenceMASMTGGQQMGRGHHHHHHGNLYFQGGEFELKVFEVHVRPKKLAVEPKGSLEVNCSTTCNQPEVGGLETSLDKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQ

Directions for Use

Download the full PDF version or continue reading below:

Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

  1. Thaw ICAM2 and dilute to desired concentration using serum-free medium or PBS. The final solutionshould be sufficiently dilute so that the volume added covers the surface evenly. Note: Use 1 ml PBS perwell in a 6-well plate.
  2. Add 1 – 10 µg protein to each well and incubate at 2 to 10°C overnight.
  3. After incubation, aspirate remaining material.
  4. Plates are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

Coating this recombinant protein at 1-10 ug / well (6 well plate) in neuronal cell specific medium can be used for1) human lymphocyte cell / receptor interaction studyin vitroand 2) as a culture matrix protein for anti-tumor immuno-response studyin vitro.

Product References

References for Lifeink®collagen bioinks:

Lee, A.et al.3D bioprinting of collagen to rebuild components of the human heart.Science365,482–487 (2019).

Maxson, Eva L., et al. "In vivo remodeling of a 3D-Bioprinted tissue engineered heart valve scaffold."Bioprinting(2019): e00059.

Filardo, G.et al.Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold.Bone & Joint Research8,101–106 (2019).

Schmitt, T. Analysis and Classification of 3-D Printed Collagen-Bioglass Matrices for Cellular Growth Utilizing Artificial Neural Networks.University Thesis (2018).

Balakhovsky, Y. M., Ostrovskiy, A. Y. & Khesuani, Y. D. Emerging Business Models Toward Commercialization of Bioprinting Technology.3D Printing and Biofabrication1–22 (2017). doi:10.1007/978-3-319-40498-1_25-1

Fox, S.et al.A simplified fabrication technique for cellularized high-collagen dermal equivalents.Biomedical Materials14,041001 (2019).

Product Certificate of Analysis

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

品牌介绍
美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。 以下为Advanced BioMatrix 3D Matrices 产品竞争优势: 1. 提供高纯度和成分确定的胞外基质; 2. 超过1000余篇文献引用PureCol产品,品质非常均一; 3. 在3D培养基领域可提供最全面的产品线; 4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft); 5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养); 6. 如果客户首次接触3D胶原凝胶,Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。 以下产品为Advanced BioMatrix全球畅销品: 1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML 2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML 3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML 4. VitroCol 人源I型胶原蛋白 #5007-20ML 5. 弹性蛋白原 #5052-1MG 6. ECM Select Array kit Ultra-36 #5170-1EA 7. CytoSoft(刚性可变的基底,Advanced BioMatrix最新添加产品5190-7EA) 8. 人III型胶原蛋白 #5021-10MG 9. 人IV型胶原蛋白 #5022-5MG 10. Silk Fibroin溶液 #5154-20ML 11. Fibronectin #5080-5MG 12. Vitronectin #5051-0.1MG Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。