4000-520-616
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BioMatrix(优势品牌)
主营:胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
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4000-520-616
当前位置: 首页 > 产品中心 > Electrophysiological > 高级生物基质/E-钙粘蛋白(CD324)//5085-0.1MG
商品详细高级生物基质/E-钙粘蛋白(CD324)//5085-0.1MG
高级生物基质/E-钙粘蛋白(CD324)//5085-0.1MG
高级生物基质/E-钙粘蛋白(CD324)//5085-0.1MG
商品编号: 5085-0.1MG
品牌: BioMatrix
市场价: ¥4400.00
美元价: 2640.00
产地: 美国(厂家直采)
公司:
产品分类: 电生理设备
公司分类: Electrophysiological
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

ProductDescription

HumanE-Cadherin(CDH1)isan882aminoacidprotein(1-23=signaldomain)thatbelongstoamemberofthecadherinsuperfamily.Theencodedproteinisacalciumdependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.Mutationsinthisgenearecorrelatedwithgastric,breast,colorectal,thyroidandovariancancer.Lossoffunctionisthoughttocontributetoprogressionincancerbyincreasingproliferation,invasion,and/ormetastasis.Theectodomainofthisproteinmediatesbacterialadhesiontomammaliancellsandthecytoplasmicdomainisrequiredforinternalization.RecentpublicationfromDingSheng’sgroupindicatedthatcoatedrecombinanthumanE-cadherinprotein(extracellulardomain)alonebenefitshumanEScellCulture.

RecombinanthumanE-Cadheringene(155-710aaFragment)wasconstructedwithcodonoptimizationandexpressedinnon-fusionproteinforminE.coliasinclusionbodies.

Thefinalproductwasrefoldedusingaunique“temperatureshiftinclusionbodyrefolding”technologyandchromatographicallypurified.

Parameter,Testing,andMethodE-Cadherin#5085
Quantity0.1mg
Volume0.2mL
Concentration0.5mg/mL
Purity-SDSPAGEElectrophoresis>90%
FormulationFormulatedin20mMpH8.0TRIS-HCLBuffer,withproprietaryformulationofNaCl,KCl,EDTA,L-Arginine,DTTandGlycerol
FormSolution
ProductionTypeRecombinant-E.Coli
StorageTemperature-20°C
ShelfLifeMinimumof6monthsfromdateofreceipt
SterilizationMethodFiltration
CellAssayPass
Sterility-USPmodifiedNoGrowth
AccessionNumberNP_004351.1
RecombinantProteinSequenceMDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIP

DirectionsforUse

DownloadthefullPDFversionorcontinuereADIngbelow:

Usetheserecommendationsasguidelinestodeterminetheoptimalcoatingconditionsforyourculturesystem.

  1. ThawE-Cadherinanddilutetodesiredconcentrationusingserum-freemediumorPBS.Thefinalsolutionshouldbesufficientlydilutesothatthevolumeaddedcoversthesurfaceevenly.Note:Use1mlPBSperwellina6-wellplate.
  2. Add5–10µgproteintoeachwellandincubateat2to10°Covernight.
  3. Afterincubation,aspirateremainingmaterial.
  4. Platesarereadyforuse.Theymayalsobestoredat2-8°Cdamporairdriedifsterilityismaintained.

E-Cadherinhasbeenusedascoatingmatrixproteinfor1)maintaininglong-termESoriPScellculturethencombinewithproperEScellculturemedia,2)asacoatingmatrixmaterialfor11RtagrecombinantTFintracellulardeliveryforproteinderivediPSprotocolwithextremelylow-levelnon-specificinteractionand3)asanativeantigenforantibodyproduction.

ProductCertificateofAnalysis

Noresultfor.

SafetyandDocumentation

SafetyDataSheet

CertificateofOrigin

ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

品牌介绍
美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。 以下为Advanced BioMatrix 3D Matrices 产品竞争优势: 1. 提供高纯度和成分确定的胞外基质; 2. 超过1000余篇文献引用PureCol产品,品质非常均一; 3. 在3D培养基领域可提供最全面的产品线; 4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft); 5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养); 6. 如果客户首次接触3D胶原凝胶,Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。 以下产品为Advanced BioMatrix全球畅销品: 1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML 2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML 3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML 4. VitroCol 人源I型胶原蛋白 #5007-20ML 5. 弹性蛋白原 #5052-1MG 6. ECM Select Array kit Ultra-36 #5170-1EA 7. CytoSoft(刚性可变的基底,Advanced BioMatrix最新添加产品5190-7EA) 8. 人III型胶原蛋白 #5021-10MG 9. 人IV型胶原蛋白 #5022-5MG 10. Silk Fibroin溶液 #5154-20ML 11. Fibronectin #5080-5MG 12. Vitronectin #5051-0.1MG Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。