ProductDescription
HumanE-Cadherin(CDH1)isan882aminoacidprotein(1-23=signaldomain)thatbelongstoamemberofthecadherinsuperfamily.Theencodedproteinisacalciumdependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.Mutationsinthisgenearecorrelatedwithgastric,breast,colorectal,thyroidandovariancancer.Lossoffunctionisthoughttocontributetoprogressionincancerbyincreasingproliferation,invasion,and/ormetastasis.Theectodomainofthisproteinmediatesbacterialadhesiontomammaliancellsandthecytoplasmicdomainisrequiredforinternalization.RecentpublicationfromDingSheng’sgroupindicatedthatcoatedrecombinanthumanE-cadherinprotein(extracellulardomain)alonebenefitshumanEScellCulture.
RecombinanthumanE-Cadheringene(155-710aaFragment)wasconstructedwithcodonoptimizationandexpressedinnon-fusionproteinforminE.coliasinclusionbodies.
Thefinalproductwasrefoldedusingaunique“temperatureshiftinclusionbodyrefolding”technologyandchromatographicallypurified.
| Parameter,Testing,andMethod | E-Cadherin#5085 |
| Quantity | 0.1mg |
| Volume | 0.2mL |
| Concentration | 0.5mg/mL |
| Purity-SDSPAGEElectrophoresis | >90% |
| Formulation | Formulatedin20mMpH8.0TRIS-HCLBuffer,withproprietaryformulationofNaCl,KCl,EDTA,L-Arginine,DTTandGlycerol |
| Form | Solution |
| ProductionType | Recombinant-E.Coli |
| StorageTemperature | -20°C |
| ShelfLife | Minimumof6monthsfromdateofreceipt |
| SterilizationMethod | Filtration |
| CellAssay | Pass |
| Sterility-USPmodified | NoGrowth |
| AccessionNumber | NP_004351.1 |
| RecombinantProteinSequence | MDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIP |
DirectionsforUse
DownloadthefullPDFversionorcontinuereADIngbelow:
Usetheserecommendationsasguidelinestodeterminetheoptimalcoatingconditionsforyourculturesystem.
- ThawE-Cadherinanddilutetodesiredconcentrationusingserum-freemediumorPBS.Thefinalsolutionshouldbesufficientlydilutesothatthevolumeaddedcoversthesurfaceevenly.Note:Use1mlPBSperwellina6-wellplate.
- Add5–10µgproteintoeachwellandincubateat2to10°Covernight.
- Afterincubation,aspirateremainingmaterial.
- Platesarereadyforuse.Theymayalsobestoredat2-8°Cdamporairdriedifsterilityismaintained.
E-Cadherinhasbeenusedascoatingmatrixproteinfor1)maintaininglong-termESoriPScellculturethencombinewithproperEScellculturemedia,2)asacoatingmatrixmaterialfor11RtagrecombinantTFintracellulardeliveryforproteinderivediPSprotocolwithextremelylow-levelnon-specificinteractionand3)asanativeantigenforantibodyproduction.
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ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
