
高级生物基质/CD2//5086-0.1MG
商品编号:
5086-0.1MG
品牌:
BioMatrix
市场价:
¥4400.00
美元价:
2640.00
产品分类:
电生理设备
公司分类:
Electrophysiological
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
ProductDescription
CD2isasurfaceantigenofthehumanT-lymphocytelineagethatisexpressedonallperipheralbloodTcells.ItisoneoftheearliestT-cellMarkers,beingpresentonmorethan95%ofthymocytes;itisalsofoundonsomenaturalkillercellsbutnotonBlymphocytes.
MonoclonalantibodiesdirectedagainstCD2inhibittheformationofrosetteswithsheeperythrocytes,indicatingthatCD2istheerythrocytereceptororiscloselyassociatedwithit.RecombinantCD2proteincouldbeservicedascoatingmatrixproteinforstudyhumanTcellsexpansioninvitro.
Full-lengthextracellulardomainofhumanCD2gene(25–209aa)wasconstructedwith31N-terminalT7/HistagandexpressedinE.coliasinclusionbodies.Thefinalproductwasrefoldedusingaunique“temperatureshiftinclusionbodyrefolding”technologyandchromatographicallypurified.
Parameter,Testing,andMethod | CD2#5086 |
Quantity | 0.1mg |
Volume | 0.2mL |
Concentration | 0.5mg/mL |
Purity-SDSPAGEElectrophoresis | >90% |
Formulation | Formulatedin20mMpH8.0TRIS-HCLBuffer,withproprietaryformulationofNaCl,KCl,EDTA,L-Arginine,DTTandGlycerol |
Form | Solution |
ProductionType | Recombinant-E.Coli |
StorageTemperature | -20°C |
ShelfLife | Minimumof6monthsfromdateofreceipt |
SterilizationMethod | Filtration |
CellAssay | Pass |
Sterility-USPmodified | NoGrowth |
AccessionNumber | NP_001758 |
RecombinantProteinSequence | MASMTGGQQMGRGHHHHHHGNLYFQGGEFELKEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD |
DirectionsforUse
DownloadthefullPDFversionorcontinuereADIngbelow:
Usetheserecommendationsasguidelinestodeterminetheoptimalcoatingconditionsforyourculturesystem.
- ThawCD2anddilutetodesiredconcentrationusingserum-freemediumorPBS.Thefinalsolutionshouldbesufficientlydilutesothatthevolumeaddedcoversthesurfaceevenly.Note:Coatingthisrecombinantproteinat1-10µg/well(6wellplate)inTcellspecificmediumcanbeusedforhumanTcelldifferentiationstudyinvitro.
- Addappropriateamountofdilutedmaterialtoculturesurface.
- Incubateatroomtemperatureforapproximately1–2hours.
- Aspirateremainingmaterial.
- RinseplatescarefullywithdH2O–avoidscratchingbottomsurfaceofplates.
- Platesarereadyforuse.Theymayalsobestoredat2-8°Cdamporairdriedifsterilityismaintained.
ProductCertificateofAnalysis
SafetyandDocumentation
SafetyDataSheet
CertificateofOrigin
ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
品牌介绍
美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。
以下为Advanced BioMatrix 3D Matrices 产品竞争优势:
1. 提供高纯度和成分确定的胞外基质;
2. 超过1000余篇文献引用PureCol产品,品质非常均一;
3. 在3D培养基领域可提供最全面的产品线;
4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft);
5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养);
6. 如果客户首次接触3D胶原凝胶,Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。
以下产品为Advanced BioMatrix全球畅销品:
1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML
2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML
3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML
4. VitroCol 人源I型胶原蛋白 #5007-20ML
5. 弹性蛋白原 #5052-1MG
6. ECM Select Array kit Ultra-36 #5170-1EA
7. CytoSoft(刚性可变的基底,Advanced BioMatrix最新添加产品5190-7EA)
8. 人III型胶原蛋白 #5021-10MG
9. 人IV型胶原蛋白 #5022-5MG
10. Silk Fibroin溶液 #5154-20ML
11. Fibronectin #5080-5MG
12. Vitronectin #5051-0.1MG
Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。
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