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BioMatrix(优势品牌)
主营:胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
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当前位置: 首页 > 产品中心 > Electrophysiological > 高级生物基质/海绵糖®//5135-5EA(直径21 mm的圆盘)
商品详细高级生物基质/海绵糖®//5135-5EA(直径21 mm的圆盘)
高级生物基质/海绵糖®//5135-5EA(直径21 mm的圆盘)
高级生物基质/海绵糖®//5135-5EA(直径21 mm的圆盘)
商品编号: 5135-5EA(21mmDiameterDiscs)
品牌: BioMatrix
市场价: ¥7900.00
美元价: 4740.00
产地: 美国(厂家直采)
公司:
产品分类: 电生理设备
公司分类: Electrophysiological
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Product Description

SpongeCol®is a collagen sponge with an interpenetrating, columnar porous architecture structure.SpongeCol®contains unique columnar porous network which permits cells and nutrients to flow completely through the interpenetrating pores and provides an increased surface area for cell attachment, growth and migration.

SpongeCol®is composed of highly purified Type I collagen which best supports the attachment, proliferation, and function of cells. The collagen is lightly cross-linked for increased mechanical strength and durability for short and long term tissue culture yet is still biodegradable over the longer-term. The diameter of the pores ranges from 100 to 400 micron with the average diameter being approximately 200 microns. The collagen disc is approximately 4 mm or 21 mm in diameter and 1.5 mm thick. The sponge discs fits into a 96-well (4 mm disc) or 12-well (21 mm disc) culture plate or sanitary luer connectors for flow perfusion. Each package contains 25 (4 mm) or 5 (21 mm) collagen sponge discs.

This product is terminally sterilized and ready-to-use.

Parameter, Testing, and MethodSpongeCol®#5135
Disc Diameter4mm or 21mm
Package Size25 or 5 Sponges/Pack
Disc Thickness1.5 mm
Pore Size~200 micron diameter with a range of 100-400 micron
Endotoxin - LAL< 1.0 EU/mL
Storage TemperatureRoom Temperature
Shelf LifeMinimum of 6 months from date of receipt
Sterilization MethodIrradiation
Sterility - USP modifiedNo growth
Collagen SourcePurified Type I Collagen
Collagen Purity - Silver Staining>99%

Directions for Use

Download the full PDF forSpongeCol® 4mm discsorSpongeCol® 21mm discs,or continue reading below:

A. Preparation and Seeding:

Note: Cell attachment to the sponge is generally the most critical step in tissue culture. Temperature, pH, gas exchange and cell concentration can affect the rate and efficiency of attachment. Optimum seeding rate depends on the type of cell being cultured.

  1. Aseptically remove the sponge discs from the packaging in a laminar flow work station.
  2. Carefully place the sponges into the wells of a 12 well (21mm sponge) or 96 well (4mm sponge) tissue culture plate using a sterile instrument. Be careful not to damage the sponge as it is being transferred. It is recommended to use non-treated tissue culture plasticware.Note: Tissue-coated plasticware may need to be coated with agarose to prevent cell attachment to the plastic instead of the sponge.
  3. Suspend cells at desired concentration (1×104– 1×105cells/mL) and dispense sufficient volume of cell solution on top of the sponge placed in the well. Note: An alternative method is to suspend cells in a neutralized collagen solution (such as PureCol® type I collagen Catalog #5005-100ML or PureCol® EZ Gel Catalog #5074-35ML). Dispense collagen/cell solution on top of the sponge placed in the well.
  4. Transfer to a 37ºC incubator for about 1 – 2 hours to allow for initial cell attachment.
  5. After 1 – 2 hour, remove the plate from the incubator and check for cell attachment. Additional testing may be required to optimize the time it takes for the cells to attach to the sponge. Check the morphology of the cells. Cell adherence and spreading will dictate the time for attachment.
  6. Once the cells have adequately attached to the sponge, increase the final volume in each well to fully cover and provide adequate medium for the culture system.

B. Changing the Media:

  1. Change the media 12 to 24 hours after the initial seeding. The frequency of changes will be determined by cell type, cell attachment efficiency, pH (maintain at pH 7.0 to 7.4) utilization of medium nutrients available to cultures. More frequent medium changes may be required compared to 2D culture systems.

C. Harvesting of Cells:

Note: Protease digestion is the standard method of releasing cells from the sponges. The strength of the attachment of the cells to the collagen sponges will vary from cell line to cell line. The enzyme concentration and digestion time will vary depending upon the activity of the enzyme and the confluence of the cells. Collagenase and/or trypsin may be the preferred method.

  1. Washing the sponge with EDTA-PBS may assist the protease digestion. Add sufficient volume to cover the sponge.
  2. Aspirate the EDTA-PBS solution from the well.
  3. Add sufficient dissociation solution to the well to fully over the sponge.
  4. Transfer to a 37ºC incubator. Check for cell detachment periodically for cell detachment.
  5. Once the cells have fully detached, remove the cells and dispense in a centrifuge tube.
  6. Centrifuge the cells as require.

Product Cell Assay

Endothelial Fibroblasts at 100X magnification. Images taken after 8 days of cell culture within SpongeCol®. Cells stained with Endothelial RSP.

Product References

References for SpongeCol®:

1. Mak, W. C., et al. “Thermo-Rheological Responsive Microcapsules for Time-Dependent Controlled Release of Human Mesenchymal Stromal Cells.”Biomater. Sci., vol. 5, no. 11, 2017, pp. 2241–2250., doi:10.1039/c7bm00663b.

2. Udhayakumar, Sivalingam, et al. “l-Arginine Intercedes Bio-Crosslinking of a Collagen–Chitosan 3D-Hybrid Scaffold for Tissue Engineering and Regeneration: in Silico, in Vitro, and in Vivo Studies.”RSC Advances, vol. 7, no. 40, 2017, pp. 25070–25088., doi:10.1039/c7ra02842c.

3. Ferreira, L.p., et al. “Design of Spherically Structured 3D in Vitro Tumor Models -Advances and Prospects.”Acta Biomaterialia, 2018, doi:10.1016/j.actbio.2018.05.034.

4. Hatsell, Sarah J., et al. “ACVR1 R206H Receptor Mutation Causes Fibrodysplasia Ossificans Progressiva by Imparting Responsiveness to Activin A.”Science Translational Medicine, vol. 7, no. 303, Feb. 2015, doi:10.1126/scitranslmed.aac4358.

5. Anton-Sales, I., Beekmann, U., Laromaine, A., Roig, A. & Kralisch, D. Opportunities of Bacterial Cellulose to Treat Epithelial Tissues.Current Drug Targets20,808–822 (2019).

Product Certificate of Analysis

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Product Videos

link to library blog - SpongeCol<sup>®</sup> Collagen Sponge
SpongeCol® Collagen Sponge

Video

link to library blog - PureCol<sup>®</sup> EZ Gel - Easy 3D Collagen Gels
PureCol® EZ Gel - Easy 3D Collagen Gels

Video

link to library blog - Seeding Collagen Gels with Cells
Seeding Collagen Gels with Cells

Video

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Safety and Documentation

Safety Data Sheet

Certificate of Origin 4mm

Certificate of Origin 21mm

Declaration of Material Source

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

品牌介绍
美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。 以下为Advanced BioMatrix 3D Matrices 产品竞争优势: 1. 提供高纯度和成分确定的胞外基质; 2. 超过1000余篇文献引用PureCol产品,品质非常均一; 3. 在3D培养基领域可提供最全面的产品线; 4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft); 5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养); 6. 如果客户首次接触3D胶原凝胶,Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。 以下产品为Advanced BioMatrix全球畅销品: 1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML 2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML 3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML 4. VitroCol 人源I型胶原蛋白 #5007-20ML 5. 弹性蛋白原 #5052-1MG 6. ECM Select Array kit Ultra-36 #5170-1EA 7. CytoSoft(刚性可变的基底,Advanced BioMatrix最新添加产品5190-7EA) 8. 人III型胶原蛋白 #5021-10MG 9. 人IV型胶原蛋白 #5022-5MG 10. Silk Fibroin溶液 #5154-20ML 11. Fibronectin #5080-5MG 12. Vitronectin #5051-0.1MG Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。