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BioMatrix(优势品牌)
主营:胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
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当前位置: 首页 > 产品中心 > Gel_imager > 高级生物基质/Gelin-S//GS231-5EA(5个单位x 1毫升)
商品详细高级生物基质/Gelin-S//GS231-5EA(5个单位x 1毫升)
高级生物基质/Gelin-S//GS231-5EA(5个单位x 1毫升)
高级生物基质/Gelin-S//GS231-5EA(5个单位x 1毫升)
商品编号: GS231-5EA(5unitsx1mL)
品牌: BioMatrix
市场价: ¥3200.00
美元价: 1920.00
产地: 美国(厂家直采)
公司:
产品分类: 凝胶成像仪
公司分类: Gel_imager
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

ProductDescription

Gelin-S®isthiol-modifiedgelatin(denaturedcollagen)andisacomponentoftheHyStem®-C,andHyStem-HPhydrogelkits.MostcellsdonotgrowwellonGelin-S–onlyhydrogels.Instead,Gelin-SshouldbeusedinconjunctionwithGlycosil®(thiol-modifiedhyaluronicacid)orHeprasil®(thiol-modifiedhyaluronicacidwiththiol-modifiedheparin).ReconstitutedGelin-Sremainsliquidat15to37°C.

ThegelatinusedtomakeGelin-SisfromTypeAGelatin,Bloom250,derivedfromporcineskin.

Gelin-S®(thiol-modifiedgelatin)ispackagedin5.0mLvialscontaining50mg.Vialsareblanketedbynitrogenandunderaslightvacuum.

StoreGelin-Sintheoriginalvial,unopened,at-20°Cforuptooneyear.DonotuncaptheGelin-Svialssincetheywillcrosslinkinthepresenceofoxygen.UseasyringeandneedletoaddDGWatertothevials.

Note:Itisrecommendedtoreconstituteeachvialinitsentirety.

DirectionsforUse

Gelin-SispreparedbydissolvingthelyophilizedsolidintheDGWater(oranysterile,degassed,deionizedwater).Whenreconstituted,itwillbein1xphosphatebufferedsaline(PBS),pH~7.4.TheamountofDGWaterusedfordissolutiondependsonthevial.

Gelin-Sshouldbepreparedinthefollowingmanner:

  1. AllowtheGelin-Svialtocometoroomtemperature.
  2. Underasepticconditions,usingasyringeandneedle,add1mLofDGwatertothe"1mLGelin-Svial"or5mLofDGwatertothe"5mLGelin-Svial."
  3. Placethevialhorizontallyonarockerorshaker.Itwilltake<40minutesforthesolidstofullydissolve.Warmingto37°Corlessand/orgentlyvortexingwillspeedupdissolvingtime.Solutionswillbeclearandslightlyviscous.
  4. Gelin-SwillnotformahydrogelevenifExtralink®isadded.Toformahydrogel,itmustbemixedwithExtralinkandGlycosil®,orHeprasil®.

ProductQ&A

Globularparticleslessthan75kDashouldbeabletofreelydiffusethroughaHyStemhydrogel.

WhenreconstitutedusingDGwater,thepHofeachHyStemcomponentwillbeapproximately7.4-7.6.

Oneyearfromthedateofreceipt,ifstoredproperly.

Anysterile,deionized,degassedwatercanbesubstitutedforreconstitution.However,inordertoensureaccurateandpredictabledissolutionandgelationtimes,ourDGWaterishighlyrecommended,asitisdegassed,blanketedinargon,andhasundergonevalidationtestingwitheachHyStemcomponent.

Gelin-Sprovidescellularattachmentsiteswhenincorporatedinthehydrogel.Gelin-Sisthiol-modified,denaturedcollagenI,derivedfromeitherbovineorporcinesources.Gelin-SisincludedinallHyStem-CandHyStem-HPkits.

Gelin-Shasbeenthiol-modifiedinthesamemannerasthehyaluronaninGlycosil(orHeprasil),sothatitcovalentlycrosslinkswiththeExtralinkintheHyStemhydrogels.

Yes.Peptidesthatcontainacysteineresiduecanbeused.Thecysteineresiduemustbepresentforthepeptidetobecovalentlybondedtothehydrogelsubstrate.

Yes.ECMproteins,suchaslaminin,collagen,fibronectin,orvitronectincanbenon-covalentlyincorporatedintothehydrogelpriortocrosslinking.

HyStemhydrogelsandspongesdifferinhydrationandhomogeneity.HyStemspongesaretypicallypolymerizedhydrogelsthataresubsequentlyfreeze-dried.Theresultingspongeisafibrous,meshnetworkwithporesandnichesthatenablecellstoinfiltrateandadhere.AtrueHyStemhydrogelisanencapsulatingliquidthatpolymerizesaroundsUSPendedcellsinculture.

No.ThecomplianceofthehydrogelsissetbytheamountofExtralinkcrosslinkeradded,theconcentrationofGlycosil(orHeprasil)andGelin-Sused,andtheratioofGlycosil(orHeprasil)toGelin-S.Oncethischemicalstructureofthehydrogelisfixed,itisnotalteredbyprolongedexposuretocellculturemedium.

HyStemspongescanbeterminallysterilizedbyE-beam.HyStemhydrogelshavenotyetbeenvalidatedforusewithE-beamsterilizationmethods.HyStemhydrogelsarenotterminallysterilizedbygammairrADIation.

Gelationtimeisaffectedbymultipleaspectsofthegel’scomposition.Onewaytochangethegelationtimeofahydrogelistovarytheamountofcrosslinkerused.GelswithaloweramountofExtralinkcrosslinkerwillhavealongergelationtimethanthosewithahigheramountofcrosslinker.Changingtheamountofcrosslinkerwillproduceslightchangesingelationtime.GelationtimecanbedramaticallychangedbyvaryingtheGlycosil(orHeprasil)andGelin-Sconcentrations.ConcentratedsolutionsofGlycosil(orHeprasil)andGelin-Swillcreateasolutionwithamuchshortergelationtime.ThiscaneasilybedonebyreconstitutingthecomponentsinasmallervolumeofDGWater.Alternatively,dilutingthesecomponentsinlargervolumesofDGWaterwilldramaticallyincreasethetotaltimetoformthehydrogel.

HyStemHydrogelsarevirtuallytransparentandshouldnotinterferewithmicroscopy.

HyStemhydrogelsmaygeneratemildinflammationaspartofthebody’snaturalhealingprocessinresponsetoinjury.HyStemhydrogelsdonottriggerimmuneresponsewhenusedinvivo.(Theseproductsarenotforhumanuse)

HyStemisdegradedinvivobymatrixmetalloproteinases(Collagenases)andhyaluronidases.

Trypsin,Dipase,collagenase,andhyaluronidasehavebeenusedtohelpdetachcellsfromthesurfaceorfromwithinHyStemhydrogels.

Ingeneral,theporesizeforHyStem-CandHyStem-HPhydrogelsis~17nm.

ProductApplications

Clickonthetitleofthedesiredprotocoltolearnmore:

2DCellGrowthonHyStemHydrogels

HyStem3DCellEncapsulationforCellDeliveryApplicationsGuide

HyStem3DCellEncapsulationinhydrogelsusing96-wellplates

HyStem3DCellEncapsulationinhydrogelsusingTCInserts

EnzymeDigestionofHyStemHydrogelsforRecoveryofEncapsulatedCells

FluorescentLabelingofHyStemHydrogels

CellRecoveryfromSurfaceofHyStemHydrogels

HyStemECMIncorporation

HyStemGelationTimeVariation

HyStemStiffnessVariationProtocolfor7.5mLkit

HyStemStiffnessVariationProtocolfor12.5mLkit

ProductReferences

ReferencesforHyStem®:

Gaetani,R.,etal.(2015)EpicardialapplicationofcardiacProgenitorcellsina3D-printedgelatin/hyaluronicacidpatchpreservescardiacfunctionaftermyocardialinfarction.Biomaterials61:339-348.PMID:17335875.Prestwich,G.D.,etal.(2007)3-Dcultureinsyntheticextracellularmatrices:newtissuemodelsfordrugtoxicologyandcancerdrugdiscovery.AdvEnzymeRegul47:196-207.PMID:17335875.Shu,X.Z.,etal.(2006)Synthesisandevaluationofinjectable,insitucrosslinkablesyntheticextracellularmatricesfortissueengineering.JBiomedMaterResA79:901-912.PMID:16941590.Shu,X.Z.,etal.(2003)Disulfide-crosslinkedhyaluronan-gelatinhydrogelfilms:acovalentmimicoftheextracellularmatrixforinvitrocellgrowth.Biomaterials24:3825-3834.PMID:12818555.

S.Cai,etal.(2005)Injectableglycosaminoglycanhydrogelsforcontrolledreleaseofhumanbasicfibroblastgrowthfactor.Biomaterials,26,6054-6067.D.B.Pike,etal.(2006)Heparin-regulatedreleaseofgrowthfactorsinvitroandangiogenicresponseinvivotoimplantedhyaluronanhydrogelscontainingVEGFandbFGF.Biomaterials,27,5242–5251.G.D.Prestwich,etal.(2007)3-DCultureinSyntheticExtracellularMatrices:NewTissueModelsforDrugToxicologyandCancerDrugDiscovery.invited,Adv.Enz.Res.,inpress(2007).X.Z.Shu,etal,(2006)SynthesisandEvaluationofInjectable,InSituCrosslinkableSyntheticExtracellularMatrices(sECMs)forTissueEngineering.J.BiomedMater.Res.A,79A(4),901-912.

Shu,X.Z.,etal.(2004)Insitucrosslinkablehyaluronanhydrogelsfortissueengineering.Biomaterials25:1339-1348.PMID:14643608.Mehra,T.D.,etal.(2006)Molecularstentingwithacrosslinkedhyaluronanderivativeinhibitscollagengelcontraction.JInvestDermatol126:2202-2209.PMID:16741511.Shu,X.Z.,etal.(2004)AttachmentandspreadingoffibroblastsonanRGDpeptide-modifiedinjectablehyaluronanhydrogel.JBiomedMaterResA68:365-375.PMID:14704979.Ghosh,K.,etal.(2007)CelladaptationtoaphysiologicallyrelevantECMmimicwithdifferentviscoelasticproperties.Biomaterials28:671-679.PMID:17049594.

ProductCertificateofAnalysis

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SafetyandDocumentation

CertificateofOrigin

SafetyDataSheet

ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

品牌介绍
美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。 以下为Advanced BioMatrix 3D Matrices 产品竞争优势: 1. 提供高纯度和成分确定的胞外基质; 2. 超过1000余篇文献引用PureCol产品,品质非常均一; 3. 在3D培养基领域可提供最全面的产品线; 4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft); 5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养); 6. 如果客户首次接触3D胶原凝胶,Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。 以下产品为Advanced BioMatrix全球畅销品: 1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML 2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML 3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML 4. VitroCol 人源I型胶原蛋白 #5007-20ML 5. 弹性蛋白原 #5052-1MG 6. ECM Select Array kit Ultra-36 #5170-1EA 7. CytoSoft(刚性可变的基底,Advanced BioMatrix最新添加产品5190-7EA) 8. 人III型胶原蛋白 #5021-10MG 9. 人IV型胶原蛋白 #5022-5MG 10. Silk Fibroin溶液 #5154-20ML 11. Fibronectin #5080-5MG 12. Vitronectin #5051-0.1MG Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。