高级生物矩阵/ECM选择<sup>®</sup>阵列套件Ultra-36//5170-1EA-免疫在线蚂蚁淘旗下平台

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商品详细高级生物矩阵/ECM选择^®阵列套件Ultra-36//5170-1EA

高级生物矩阵/ECM选择^®阵列套件Ultra-36//5170-1EA

商品编号： 5170-1EA

品牌： BioMatrix

市场价： ¥5600.00

美元价： 3360.00

产地：美国(厂家直采)

公司：

产品分类： ELISA辅助试剂

公司分类： ELISA_helper_reagent

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

ProductDescription

ECMSelect^®ArrayKitUltra-36isanextracellularmatrixscreeningarray.Thisproducthasthirtysix(36)humanextracellularmatrixprotein(ECM)conditionsthataredepositedontothehydrogelsurfaceasprintedarrayspots.EachECMconditionisprintedwithnine(9)replicatespotswhereeachspothasdiameterof400µm.TheECMsarelocalizedwithineachspotwithoutcrossdiffusionfromneighboringspots,thereforeeachspotrepresentsanindependent‘well’orexperiment.

TheECMSelect^®ArrayKitUltra-36containsamicroscopeglassslideprintedwiththeECMconditionscontainedwithinaprotectiveslideholderandapre-sterilizedtray.Thecomponentsaresterilizedandready-to-use.

CellsofinterestareseededontotheECMSelect^®Arrayslideandallowedtobeculturedintheincubatorforthedesiredamountoftime.Cellmorphology,attachmentandgrowthcanbevisualizedusingabrightfieldmicroscope.SpecificcellularbehaviorscanbemonitoredbystainingthecellsontheslideusingspecificfluorescencebasedMarker.Fluorescencesignalcanbedetectedusingfluorescencemicroscopeimagingsystem.

TheECMSelect^®ArrayKitUltra-36issimpletouseincomparisontoperformingthesameexperimentinmulti-welltissuecultureplates.Cellseeding,fixing,washingandprocessstepsaredoneinonesinglesolutionexchange,eliminatingmulti-wellsolutionhandlings.Fromstarttofinish,theoptimalECMforculturingcellscanbedeterminedinlessthan24hoursformostcelltypes.

DirectionsforUse

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ProductQ&A

Becauseitiscontactprinting,itishardtodeterminethethepreciseamount.Basedonproteinstainingresult,eachspotisabout0.1-1ng.

TheremaybepossibilityofantagoNISTeffectoftwodifferentcomponent.Wehavenotseenthisveryoften,whatweusuallyseeisoneofthecomponentbyitselfdoesnotprovideadhesioneffect,butwhencombinewithanotherECM,greatlyenhancetheotherECM.

Thestiffnessis~10kPa.

MountingmediatendstodestroythepolyacrylamidehydrogelthattheECMspotsareprintedonto.Thebestmethodwehavefoundistoincubatetheentireslidefor20minuteswith4%PFApriortothestainprotocol.Theslidewascover-slippedwitha90%glycerolmediumcontainingthePPDanti-fadecompoundandsealedwithfingernailpolisharoundtheedges.

ProductReferences

ReferencesforECMSelect^®:

Shaheen,NoelL.,etal."Extracellularmatrixcompositionmodulatesangiosarcomacellattachmentandproliferation."Oncoscience11-12(2017):178.

Ferreira,L.P.,V.M.Gaspar,andJ.F.Mano."DesignofSphericallyStructured3DInvitroTumorModels-AdvancesandProspects."ActaBiomaterialia(2018).

ProductCertificateofAnalysis

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ProductVideos

link to library blog - ECM Select<sup>®ArrayKit

ECMSelect^®ArrayKit

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SafetyandDocumentation

SafetyDataSheet

CertificateofOrigin

ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

品牌介绍

美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量，并保证产品之间一致性，方便研究客户使用。以下为Advanced BioMatrix 3D Matrices 产品竞争优势： 1. 提供高纯度和成分确定的胞外基质； 2. 超过1000余篇文献引用PureCol产品，品质非常均一； 3. 在3D培养基领域可提供最全面的产品线； 4. 唯一可提供特异性刚性有机硅基板的公司（CytoSoft）; 5. 唯一可提供可溶性丝纤蛋白的供应商（可运用于多种3D培养）； 6. 如果客户首次接触3D胶原凝胶，Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。以下产品为Advanced BioMatrix全球畅销品： 1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML 2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML 3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML 4. VitroCol 人源I型胶原蛋白 #5007-20ML 5. 弹性蛋白原 #5052-1MG 6. ECM Select Array kit Ultra-36 #5170-1EA 7. CytoSoft（刚性可变的基底，Advanced BioMatrix最新添加产品5190-7EA） 8. 人III型胶原蛋白 #5021-10MG 9. 人IV型胶原蛋白 #5022-5MG 10. Silk Fibroin溶液 #5154-20ML 11. Fibronectin #5080-5MG 12. Vitronectin #5051-0.1MG Advanced BioMatrix最畅销的产品是PureCol（5005-100ML）。目前已有上千发表的论文使用这款产品。（Nutragen 5010、fibricol 5133和PureCol的物质成分一样，仅仅是浓度不同）浓度越高，胶原蛋白硬度越强。

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