Product Description
| Parameter, Testing, and Method | Fibronectin#5050 |
| Quantity | 1.0 mg |
| Volume | 2.0 mL |
| Concentration | 0.5 mg/mL |
| Purity - SDS PAGE Electrophoresis | >95% |
| Formulation | 20 mM Tris-HCL, pH 7.0 containing 0.45 M NaCl and 12% Glycerol |
| Form | Solution |
| Source | Human, Plasma |
| Storage Temperature | -20°C |
| Shelf Life | Minimum of 6 months from date of receipt |
| Sterilization Method | Filtration |
| Cell Attachment Assay | Passes |
| Sterility - USP modified | No growth |
| Safety | Source material found negative for infectious agents |
Directions for Use
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Coating Procedures:
Use these recommendations as guidelines todetermine the optimal coating conditions for yourculture system.
- Thaw Fibronectin and dilute to desiredconcentration using serum-free medium orPBS (Ca++, Mg++ free). The final solutionshould be sufficiently dilute so that thevolume added covers the surface evenly.
- Add appropriate amount of dilutedmaterial to culture surface.
- Incubate at room temperature forapproximately 1 hour.
- Aspirate remaining material.
- Rinse plates carefully with dH2O– avoidscratching bottom surface of plates.
- Plates are ready for use. They may alsobe stored at 2-8°C damp or air dried ifsterility is maintained.
Product Q & A
440 kDa. The protein is a dimer of two 220 kDa identical subunits. It shows up as 220 kDa on a reduced gel, and 440 kDa on a non-reduced gel.
Product References
References for Fibronectin:
Alhussein, Ghada, et al. "A spatiotemporal characterization method for the dynamic cytoskeleton."Cytoskeleton73.5 (2016): 221-232.
Pezzoli, Daniele, et al. "Fibronectin promotes elastin deposition, elasticity and mechanical strength in cellularised collagen-based scaffolds."Biomaterials180 (2018): 130-142.
Wiley, Luke A., et al. "Generation of xeno‐free, cGMP‐compliant patient‐specific iPSCs from skin biopsy."Current protocols in stem cell biology42.1 (2017): 4A-12.
Wiley, Luke A., et al. "Using patient-specific induced pluripotent stem cells and wild-type mice to develop a gene augmentation-based strategy to treat CLN3-associated retinal degeneration."Human gene therapy27.10 (2016): 835-846.
Worthington, Kristan S., et al. "Two-photon polymerization for production of human iPSC-derived retinal cell grafts."Acta biomaterialia55 (2017): 385-395.
Li, Ling, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn. "Isolation and cultivation of dermal stem cells that differentiate into functional epidermal melanocytes."Human Cell Culture Protocols. Humana Press, 2012. 15-29.
Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle."Journal of tissue engineering and regenerative medicine12.2 (2018): e918-e936.
Product Certificate of Analysis
Safety and Documentation
Safety Data Sheet
Certificate of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
