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BioMatrix(优势品牌)
主营:胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
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4000-520-616
当前位置: 首页 > 产品中心 > plate > 高级BioMatrix/CytoSoft®成像96孔板//5258-1EA 8 kPa
商品详细高级BioMatrix/CytoSoft®成像96孔板//5258-1EA 8 kPa
高级BioMatrix/CytoSoft®成像96孔板//5258-1EA 8 kPa
高级BioMatrix/CytoSoft®成像96孔板//5258-1EA 8 kPa
商品编号: 5258-1EA8kPa
品牌: BioMatrix
市场价: ¥3300.00
美元价: 1980.00
产地: 美国(厂家直采)
公司:
产品分类:
公司分类: plate
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

ProductDescription

ThisCytoSoft®Imaging96-wellproducthasadefinedelasticmodulus(seecertificateofanalysis)withaglassbottomforhighresolutionimagingThethicknessofthesiliconegelisuniformwitha~0.03mmthicklayerofsiliconeineachwell.ThesiliconegelsareactivatedandreadytobindtoapurifiedECM,suchasPureCol®typeIcollagen(#5005)priortocelladdition.

Therigidityofthesubstratetowhichcellsadherecanhaveaprofoundeffectoncellmorphologyandgeneexpression.CytoSoft®productsprovideatooltoculturecellsonsubstrateswithvariousrigiditiescoveringabroadphysiologicalrangeusingathinlayerofbiocompatIBLesilicone.

TheCytoSoft®–Imagingplatehasaglassbottomforhigh-resolutionimagingwherelowautofluorescenceandexceptionalopticalclarityarerequired.The96-wellformatisoptimalforhigh-throughputcellstudiesandscreening.Theplateconsistsofa#1.5thicknessglassbottombondedtoablackpolystyreneframeandincludesalid.Theplatesaresterilizedusingozoneandprovidedwith1plateperpackage.

Onthebottomofeachwell,thereisathinlayerofspeciallyformulatedbiocompatiblesilicone,whoseelasticmodulus(rigidity)iscarefullymeasuredandcertified.ThesurfacesofthegelsinCytoSoft®productsarefunctionalizedtoformcovalentbondswithaminesonproteins.Thechemicalfunctionalizationisstableandthereactiondoesnotrequireacatalyst,facilitatingcoatingofthegelsurfaceswithmatrixproteinsandplatingcells.

Thesiliconesubstratesareopticallyclearandhaveanearzeroauto-florescence.Thelayerofsiliconeineachwellisfirmlybondedtothebottomofthewell.Unlikehydrogels(suchaspolyacrylamidegels),siliconegelsarenotsusceptibletohydrolysis,donotdryorswell,areresilientandresistanttotearingorcracking,andtheirelasticmoduli(rigidities)remainnearlyunchangedduringextendedstoragetimes.

CytoSoft®-Imagingproductsaccommodatelivecellstainingusingmembraneandcellpermeabledyes;fixationofcellsusingcommontechniquessuchasparaformaldexyde;andimmunostainingoffixedcells.

Parameter,Testing,andMethodCytoSoft®Imaging96-WellPlates
SterilizationMethodOzone
PlateSize96-MultiwellBlackPolystyrenePlatewithGlassBottom
QuantityperPackage1Plate
Rigidty(ElasticModulus)0.2,0.5,2,8,16,32,64kPa(exactvaluesprovidedontheCofA)
StorageTemperatureRoomTemperature
ShelfLifeMinimumof6monthsfromdateofreceipt
PlateSurfaceMaterialFunctionalizedSilicone

GrowthAreaperWell

0.32cm2

TypicalWorkingVolumeperWell

40to280μL

CellAttachmentAssay

Pass

DirectionsforUse

DownloadthefullPDFversionorcontinuereADIngbelow:

CoatingProcedure

Note:Usetheserecommendationsasguidelinestodeterminetheoptimalcoatingconditionsforyourculturesystem.

CautionaryNote:Thebottomofa96-wellplatecanbedetachedbyexcessivemechanicalforcesuchascentrifugationordirectcontactwithliquidhandlingtools(tips,Pipettes,etc.).

RemovetheCytoSoft®productfromtheprotectivesleeveinasterilehood.

  1. Prepareextracellularmatrixmaterialbyneutralizinginamine-freebufferpH7.4to7.9(suchas1XDPBS).WedonotrecommendusinggelatinasyourECMprotein.

Note:Pre-warmthecoatingsolutiontoapproximatelyroomtemperaturebeforeuse.

  1. Diluteasneeded,anddispense~150uLofsolutionintoeachwelltofullycoatthesurface.

Note:RecommendeddilutionforPureCol®3mg/mlTypeIcollagenis1:30(~100µg/ml).

Note:Thehydrophobicsurfacerequireslargervolumestocoverthesurfacethandoconventionalplasticdishes

  1. IncubateECMcoatedCytoSoft®atroomtemperature,coveredfor0.5-1hour.
  1. Afterincubation,aspirateanyremainingmaterialandrinsecoatedsurfacesimmediatelytwotimeswithculturemediumorPBS.Leaveabout150uLofmediumperwelltokeepsurfacecovered.

Note:DonotallowtheCytoSoft®surfacetobecomedryoncethesurfacehasbeenwetted.

  1. Coatedsurfacesarereadyforuse.
  2. StandardharvestingproceduresusedforremovingcellsfromculturewarecanbeemployedforharvestingcellsfromtheCytoSoft®productincludinguseoftrypsin,Accutase®andnon-enzymaticcelldetachmentsolutions.

ProductQ&A

Werecommenda60Xobjectivefortheimagingplates.

Theelasticmodulusismeasuredbytrackingbeadsonthegelsurfaceunderawide-fieldfluorescencemicroscopewithoutanyotherspecializedequipment.Themeasurementshavesmallandsimpletoestimateerrorsandtheirresultsareconfirmedbyconventionaltensiletests.AmastercurveisobtainedrelatingthemixingratiosofthetwocomponentsofSylgard184withtheresultingelasticmoduliofthegels.

Usingprewarmedmediawilldecreasegassolubilityandhelppreventbubblesonthesurfaceofthesilicone.

Thesiliconegelcancrackifthesurfacebecomesdry.

No.Cellmatrixproteinsareattachedtothesurfaceofthegelviacovalentbonding.ItisdifficulttoformanewECMlayeraftercelldetachment.Therearenolongeranyreactivegroupsonthesurfaceofthegelaftertheinitialcellculture.

Thechangeintherefractiveindexofthesiliconedistortsthingsalittle,soDICwillbepossiblebutnotperfect.Also,DICisonlypossibleontheglassbottomimagingplates,notthe6-wellplasticplates.

1.41

Thetwomainissuesare:

1.IneffecientcoatingofthematrixproteinsduetolowpHofthecoatingsolution.

2.Insufficientwashofleftovercellmatrixafterthecoatingprocedure.

No.CytosoftmustbecoatedwithanECMproteinsuchasCollagenorFibronectin.

Thesurfaceisdecoratedwithanhydridefunctionalgroups.

Plasmauseisnotrecommended.PlasmawillinduceformationofahardcrustonthesurfaceandwillchangethemechanicalpropertiesoftheCytoSoft®products.

DonotfreezetheCytoSoft®products.

Whenfrozen,thereisagoodchancethatthesiliconesurfacegetshydrolyzedandabsorbsmoisture,whichwouldinactivatethebindingsitesandmaketheproductnot-usable.

Ifyoufrozetheproductanditisstillfrozen,warmtheCytoSoft®upat60Cwiththebagvented.Thatwillminimizethechanceofthemabsorbingmoisture–butthereisstillachancethattheywillnolongerbefunctional.

UsinggelatinforcoatingCytoSoftisoftenproblematicbecausegelatinoftenhaslowmolecularweightimpuritiesthatblockbindingsitesontheactivatedsurfaceofthesilicone.WerecommendusinghighlypurifiedECM"sinstead.

ProductCellAssay

CytoSoft®platescanalsobeusedtoshowhowfibroblastsareabletodiscriminatebetweentheunderlyingstiffness.Thisismanifestedinbothadhesionsizeandstressfibers,asseenbelow.Itappearsthatthecellsonthe8kPastiffnesshavereducedintracellulartensionandincreasedadhesion.

ProductReferences

ReferencesforCytoSoft®Products:

1.Modaresi,Saman,etal.“DecipheringtheRoleofSubstrateStiffnessinEnhancingtheInternalizationEfficiencyofPlasmidDNAinStemCellsUsingLipid-BasedNanocarriers.”Nanoscale,vol.10,no.19,2018,pp.8947–8952.,doi:10.1039/c8nr01516c.

2.Wilson,ChristinaL.InVitroModelsOfBrainForStudyOfMolecularMechanismsInBrainDisorder.TheUniversityofNEBraska-Lincoln,2016.

3.Wilson,C.L.,Hayward,S.L.,&Kidambi,S.(2016).Astrogliosisinadish:Substratestiffnessinducesastrogliosisinprimaryratastrocytes.RSCAdvances,6(41),34447-34457.doi:10.1039/c5ra25916a

4.Prager-KhoutorskyM,LichtensteinA,KrishnanR,RajendranK,MayoA,KamZ,GeigerB,BershadskyAD.Fibroblastpolarizationisamatrix-rigidity-dependentprocesscontrolledbyfocaladhesionmechanosensing.Nat.CellBiol.2011;13:1457–1465.

5.GutierrezE,TkachenkoE,BesserA,SunddP,LeyK,DanuserG,GinsbergMH,GroismanA.HighRefractiveIndexSiliconeGelsforSimultaneousTotalInternalReflectionFluorescenceandTractionForceMicroscopyofAdherentCells.PLoSOne.2011;6:e23807.

6.MerkelR,KirchgessnerN,CesaCM,HoffmannB.Cellforcemicroscopyonelasticlayersoffinitethickness.Biophys.J.2007;93:3314–23.

7.Schellenberg,A.etal.Matrixelasticity,replicativesenescenceandDNAmethylationpatternsofmesenchymalstemcells.Biomaterials35,6351–8(2014).

8.Cesa,C.M.etal.Micropatternedsiliconeelastomersubstratesforhighresolutionanalysisofcellularforcepatterns.Rev.Sci.Instrum.78,034301(2007).

9.Gutierrez,E.&Groisman,A.MeasurementsofElasticModuliofSiliconeGelSubstratewithaMicrofluidicDevice.PlosOne6(2011).

10.Mori,H.,Takahashi,A.,Horimoto,A.,andHara,M.Migrationofglialcellsdifferentiatedfromneurosphere-formingneuralstem/Progenitorcellsdependsonthestiffnessofthechemicallycross-linkedcollagengelsubstrate.NeuroscienceLetters,Vol.555,October(2013)

11.Banerjee,I.,Carrion,K.,Serrano,R.,Dyo,J.,Sasik,R.,Lund,S.etal.Cyclicstretchofembryoniccardiomyocytesincreasesproliferation,growth,andexpressionwhilerepressingTgf-βsignaling.JMolCellCardiol.2015;79:133–144

12.Vertelov,G.etal.Rigidityofsiliconesubstratescontrolscellspreadingandstemcelldifferentiation.Sci.Rep.6,33411;doi:10.1038/srep33411(2016).

13.TkachenkoE,RawsonR,LaE,etal.RigidSubstrateInducesEsophagealSmoothMuscleHypertrophyandEoEFibroticGeneExpression.TheJournalofallergyandclinicalimmunology.2016;137(4):1270-1272.e1.doi:10.1016/j.jaci.2015.09.020.

14.Sao,K.etal.MyosinIIgovernsintracellularpressureandtractionbydistincttropomyosin-dependentmechanisms.MolecularBIOLOGyoftheCell30,1170–1181(2019).

15.Cooper,J.G.etal.SpinalCordInjuryResultsinChronicMechanicalStiffening.JournalofNeurotrauma(2019).doi:10.1089/neu.2019.6540

ProductCertificateofAnalysis

Noresultfor.

ProductVideos

link to library blog - What is CytoSoft<sup>®?
WhatisCytoSoft®?

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BDceacb54ca4b15499b4.jpg"alt="linktolibraryblog-HowtoCoatCytoSoft®Plates">
HowtoCoatCytoSoft®Plates

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SafetyandDocumentation

SafetyDataSheet

CertificateofOrigin

ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

品牌介绍
美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。 以下为Advanced BioMatrix 3D Matrices 产品竞争优势: 1. 提供高纯度和成分确定的胞外基质; 2. 超过1000余篇文献引用PureCol产品,品质非常均一; 3. 在3D培养基领域可提供最全面的产品线; 4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft); 5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养); 6. 如果客户首次接触3D胶原凝胶,Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。 以下产品为Advanced BioMatrix全球畅销品: 1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML 2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML 3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML 4. VitroCol 人源I型胶原蛋白 #5007-20ML 5. 弹性蛋白原 #5052-1MG 6. ECM Select Array kit Ultra-36 #5170-1EA 7. CytoSoft(刚性可变的基底,Advanced BioMatrix最新添加产品5190-7EA) 8. 人III型胶原蛋白 #5021-10MG 9. 人IV型胶原蛋白 #5022-5MG 10. Silk Fibroin溶液 #5154-20ML 11. Fibronectin #5080-5MG 12. Vitronectin #5051-0.1MG Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。