Product Description
Advanced BioMatrix offers PhotoCol®, a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.
The PhotoCol® kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit. Table 1 provides a list of the kit components.
Table 1:
| Item | Catalog Number | Package Size | Storage Temperature |
| Methacrylated Collagen | #5198 | 100 mg | 2-10°C |
| 20 mM Acetic Acid | #5079 | 50 mL | 2-30°C |
| Neutralization Solution | #5205 | 10 mL | Room Temperature |
| Irgacure Photoinitiator | #5200 | 100 mg | 2-10°C |
The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on theN-termini. > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content.
The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes.A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.
| Parameter, Testing, and Method | Methacrylated Collagen#5198 |
| Sterilization Method | Filtration |
| Extraction Method | Acid - Telocollagen |
| Form | Lyophilized Powder |
| Degree of Methacrylation | >20% |
| Package Size | 100 mg |
| Shelf Life | Minimum of 6 months from date of receipt |
| Shelf Life After Reconstitution | 2 Months |
Collagen Purity - Silver Staining | >99% |
| Kinetic Gel Test (Minutes) | <40 |
| Gel Formation Tube Test (Minutes) | < 40 |
Electrophoretic Pattern - Coomassie Blue | Characteristic |
| Sterility - USP modified | No growth |
| Endotoxin - LAL | <10.0 EU/mL |
| Source | Bovine Hide |
| Hydrogel Young's Modulus E (Pa) | Characteristic |
Directions for Use
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Product Applications
PhotoCol®Methacrylated Collagen is a photocrosslinkable type I collagen for 3D cell culture and bioprinting.
As the most abundant protein in the body, collagen is a key component for cell culture. Collagen methacrylate can be used as a rapidly self-assembling type I collagen to form cross-linked hydrogels for tissue engineering[1]. It has been used with mesenchymal stem cells[2], fibroblasts, adipose derived stem cells, epithelial cells, and many more.
Collagen methacrylate is useful for forming scaffolds with varying degree of stiffness, by altering collagen concentration or the dose of UV-light exposure[2]. It is also being extensively characterized for its usefulness in 3D bioprinting (extrusion, inkjet, and photolithographic[3]).
PhotoCol® has been used for created 3D microniches[4], as well as for 3D bioprinting human corneas[5].
References:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172302/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243547/
http://www.worldscientific.com/doi/abs/10.1142/S2339547817500091
https://onlinelibrary.wiley.com/doi/abs/10.1002/adbi.201700237
https://www.ncbi.nlm.nih.gov/pubmed/29772228
Product References
References for PhotoCol®:
Isaacson, A., Swioklo, S. & Connon, C. J. 3D bioprinting of a corneal stroma equivalent.Experimental Eye Research173,188–193 (2018).
Kathryn E. Drzewiecki et al, A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine,TECHNOLOGY(2017).
Drzewiecki, K. E.et al.Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen.Langmuir30,11204–11211 (2014).
Gaudet, I. D. & Shreiber, D. I. Characterization of Methacrylated Type-I Collagen as a Dynamic, Photoactive Hydrogel.Biointerphases7,25 (2012).
Izadifar, Mohammad, et al. "UV-assisted 3D bioprinting of nanoreinforced hybrid cardiac patch for myocardial tissue engineering."Tissue Engineering Part C: Methods24.2 (2018): 74-88.
Stoecklin, Celine, et al. "A New Approach to Design Artificial 3D Microniches with Combined Chemical, Topographical, and Rheological Cues."Advanced Biosystems(2018): 1700237.
Mazzocchi, Andrea, et al. "Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments."Biofabrication(2018).
Nguyen, T. U., Watkins, K. E. & Kishore, V. Photochemically crosslinked cell‐laden methacrylated collagen hydrogels with high cell viability and functionality.Journal of Biomedical Materials Research Part A107,1541–1550 (2019).
Mazzocchi, A.et al.Pleural Effusion Aspirate for Use in 3D Lung Cancer Modeling and Chemotherapy Screening.ACS Biomaterials Science & Engineering5,1937–1943 (2019).
Chhetri, A.et al.Cell Culture and Coculture for Oncological Research in Appropriate Microenvironments.Current Protocols in Chemical Biology11,(2019).
Product Certificate of Analysis
Product Videos
How to Make 3D Collagen Hydrogels
Video
Telo vs Atelo Collagen
Video
Neutralization Solution for Easy 3D Collagen Gels
Video
Seeding Collagen Gels with Cells
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4 Key Components for 3D Collagen Gels
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Safety and Documentation
Safety Data Sheet
Certificate of Origin
Declaration of Material Source
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
