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BioMatrix(优势品牌)
主营:胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
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当前位置: 首页 > 产品中心 > Electrophysiological > Advanced BioMatrix/Type V Collagen//5168-0.5MG
商品详细Advanced BioMatrix/Type V Collagen//5168-0.5MG
Advanced BioMatrix/Type V Collagen//5168-0.5MG
Advanced BioMatrix/Type V Collagen//5168-0.5MG
商品编号: 5168-0.5MG
品牌: BioMatrix
市场价: ¥6400.00
美元价: 3840.00
产地: 美国(厂家直采)
公司:
产品分类: 电生理设备
公司分类: Electrophysiological
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Product Description

Type V Collagen provides structure and strength to connective tissue and is found in many places in the body, especially skin, lung, intestinal walls and walls of blood vessels. This Type V Collagen product is isolated from placenta and is purified using a multi-step process with a final purity of >95%. Type V collagen forms a triple helix composed of aAlpha1(V)2 Alpha 2(V) heterotrimer.

The product is supplied as a sterile solution at 0.5 mg per vial at approximately 0.15 to 0.30 mg/ml in 100 mM acetic acid. A Certificate of Analysis is available with the purchase of each product. Type V collagen product is provided in a user-friendly packaging for use and storage.

Type V Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally usedin vitroas a substrate scaffold to enhance cell attachment, adherence and proliferation. Type V collagen may be used to culture a variety of cell types.

Parameter, Testing, and MethodType V Collagen#5168
Sterilization MethodFiltration
FormSolution
Package Size0.5 mg (~2 mL)
Storage Temperature2-10°C
Shelf LifeMinimum of 6 months from date of receipt

Collagen Concentration - Biuret

0.15-0.3 mg/mL
pH2.0-3.5

Electrophoretic Pattern - Coomassie Blue

Characteristic
Endotoxin - LAL<5.0 EU/mL
Cell Attachment AssayPass
SourceBovine Placenta

Directions for Use

Download the full PDF versionor continue reading below:

Coating Procedure

Note:Use these recommendations as guidelines todetermine the optimal coating conditions for your culturesystem.

  1. Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
  2. Dilute Type V Collagen with 100 mM acetic acid to ~10 µg/ml.
  3. Swirl contents gently until material is completelymixed.
  4. Add appropriate amount of diluted Type V Collagenmaterial to the culture surface ensuring that the entire surface is coated.
  5. Incubate at room temperature, covered, for 1-2 hours.
  6. After incubation, aspirate any remaining material.
  7. Rinse coated surfaces carefully with sterile medium orPBS, avoid scratching surfaces.
  8. Coated surfaces are ready for use. They may also bestored at 2-8°C damp or air dried if sterility is maintained.

Product Certificate of Analysis

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Product Videos

link to library blog - Coating Plates with Collagen
Coating Plates with Collagen

Video

link to library blog - Coating a Glass Coverslip with Collagen
Coating a Glass Coverslip with Collagen

Video

See More

Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

品牌介绍
美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。 以下为Advanced BioMatrix 3D Matrices 产品竞争优势: 1. 提供高纯度和成分确定的胞外基质; 2. 超过1000余篇文献引用PureCol产品,品质非常均一; 3. 在3D培养基领域可提供最全面的产品线; 4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft); 5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养); 6. 如果客户首次接触3D胶原凝胶,Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。 以下产品为Advanced BioMatrix全球畅销品: 1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML 2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML 3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML 4. VitroCol 人源I型胶原蛋白 #5007-20ML 5. 弹性蛋白原 #5052-1MG 6. ECM Select Array kit Ultra-36 #5170-1EA 7. CytoSoft(刚性可变的基底,Advanced BioMatrix最新添加产品5190-7EA) 8. 人III型胶原蛋白 #5021-10MG 9. 人IV型胶原蛋白 #5022-5MG 10. Silk Fibroin溶液 #5154-20ML 11. Fibronectin #5080-5MG 12. Vitronectin #5051-0.1MG Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。