Product Description
Type V Collagen provides structure and strength to connective tissue and is found in many places in the body, especially skin, lung, intestinal walls and walls of blood vessels. This Type V Collagen product is isolated from placenta and is purified using a multi-step process with a final purity of >95%. Type V collagen forms a triple helix composed of aAlpha1(V)2 Alpha 2(V) heterotrimer.
The product is supplied as a sterile solution at 0.5 mg per vial at approximately 0.15 to 0.30 mg/ml in 100 mM acetic acid. A Certificate of Analysis is available with the purchase of each product. Type V collagen product is provided in a user-friendly packaging for use and storage.
Type V Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally usedin vitroas a substrate scaffold to enhance cell attachment, adherence and proliferation. Type V collagen may be used to culture a variety of cell types.
| Parameter, Testing, and Method | Type V Collagen#5168 |
| Sterilization Method | Filtration |
| Form | Solution |
| Package Size | 0.5 mg (~2 mL) |
| Storage Temperature | 2-10°C |
| Shelf Life | Minimum of 6 months from date of receipt |
Collagen Concentration - Biuret | 0.15-0.3 mg/mL |
| pH | 2.0-3.5 |
Electrophoretic Pattern - Coomassie Blue | Characteristic |
| Endotoxin - LAL | <5.0 EU/mL |
| Cell Attachment Assay | Pass |
| Source | Bovine Placenta |
Directions for Use
Download the full PDF versionor continue reading below:
Coating Procedure
Note:Use these recommendations as guidelines todetermine the optimal coating conditions for your culturesystem.
- Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
- Dilute Type V Collagen with 100 mM acetic acid to ~10 µg/ml.
- Swirl contents gently until material is completelymixed.
- Add appropriate amount of diluted Type V Collagenmaterial to the culture surface ensuring that the entire surface is coated.
- Incubate at room temperature, covered, for 1-2 hours.
- After incubation, aspirate any remaining material.
- Rinse coated surfaces carefully with sterile medium orPBS, avoid scratching surfaces.
- Coated surfaces are ready for use. They may also bestored at 2-8°C damp or air dried if sterility is maintained.
Product Certificate of Analysis
Product Videos
Coating Plates with Collagen
Video
Coating a Glass Coverslip with Collagen
Video
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Safety and Documentation
Safety Data Sheet
Certificate of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
