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BioMatrix(优势品牌)
主营:胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
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当前位置: 首页 > 产品中心 > Electrophysiological > Advanced BioMatrix/Nutragen®//5010-50ML
商品详细Advanced BioMatrix/Nutragen®//5010-50ML
Advanced BioMatrix/Nutragen®//5010-50ML
Advanced BioMatrix/Nutragen®//5010-50ML
商品编号: 5010-50ML
品牌: BioMatrix
市场价: ¥8200.00
美元价: 4920.00
产地: 美国(厂家直采)
公司:
产品分类: 电生理设备
公司分类: Electrophysiological
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

ProductDescription

Nutragen®collagenisknownasthestandardofallcollagensforpurity(>99%collagencontent),functionality,andthemostnative-likecollagenavailable.Nutragen®isisolatedfrombovinehidessourcedfromtheonlycontrolled,closedherdintheUnitedStates.AdvancedBiomatrix’smanufacturingprocessescomplywithstringentqualitystandardsthathaveproventoyieldunsurpassedlot-to-lotconsistency.

Nutragen®collagenisapproximately97%TypeIcollagenwiththeremainderbeingcomprisedofTypeIIIcollagen.Nutragen®collagenissuppliedatapproximatelya6mg/mLconcentrationtoprovideanextremelyfirmgel.TheconcentrationforeachspecificlotisprovidedonaCertificateofAnalysisthatisavailablewiththepurchaseofeachproduct.Nutragen®issolubleatelo-collagenin0.01NHCI,therefore,thepHisapproximately2.0.

Nutragen®collagenisidealforcoatingofsurfaces,providingpreparationofthinlayersforculturingcells,oruseasasolidgel.Nutragen®collagenisprovidedina50mLvolumeandiscontainedinuser-friendlypackagingforuseandstorage.Nutragen®issterilefilteredandissuppliedasareadytousesolution.

Parameter,Testing,andMethodNutragen®TypeICollagen#5010
SterilizationMethodFiltration
ExtractionMethodEnzyme-atelocollagen
FormSolution
PackageSize50mL,1000mL
StorageTemperature2-10°C
ShelfLifeMinimumof6monthsfromdateofreceipt

CollagenConcentration-Biuret

5.8-6.8mg/mL

CollagenPurity-SilverStaining

>99%
pH1.9-2.1
KineticGelTest(Minutes)<40
GelFormationTubeTest(Minutes)<40

Fibrillogenesis(AbsorbanceUnits)

>0.5

ElectrophoreticPattern-CoomassieBlue

Characteristic
Sterility-USPmodifiedNogrowth
Endotoxin-LAL<1.0EU/mL
Osmolality(mOsmoH2O/kg)<35
CellAttachmentAssayPass
SourceBovineHide
HydrogelYoung"sModulusE(Pa)Characteristic

DirectionsforUse

DownloadthefullPDFversionorcontinuereADIngbelow:

CoatingProcedure

Note:Usetheserecommendationsasguidelinestodeterminetheoptimalcoatingconditionsforyourculturesystem.

  1. Removerequiredquantityofcollagenfromthebottleanddispenseintoadilutionvessel.
  2. DiluteNutragen®inwaterto~50to100µg/ml(~1:30).A0.01NHClsolutionmayalsobeused.
  3. Swirlcontentsgentlyuntilmaterialiscompletelymixed.
  4. AddappropriateamountofdilutedNutragen®materialtotheculturesurfaceensuringthattheentiresurfaceiscoated.
  5. Incubateatroomtemperature,covered,for1-2hours.Aspirateanyremainingmaterial.Alternatively,incubateatroomtemperatureuntilsurfaceisdry.
  6. Afterincubation,aspirateanyremainingmaterial.
  7. RinsecoatedsurfacescarefullywithsterilemediumorPBS,avoidscratchingsurfaces.
  8. Coatedsurfacesarereadyforuse.Theymayalsobestoredat2-8°Cdamporairdriedifsterilityismaintained.

3-DGelPreparationProcedure

  1. Slowlyadd1partofchilled10XPBSor10Xculturemediato8partsofchilledcollagensolutionwithgentleswirling.
  2. AdjustpHofmixtureto7.0–7.5.Useofsterile0.1MNaOHisrecommended.MonitorpHadjustmentcarefully(pHmeter,phenolred,orpHpaper).
  3. Adjustfinalvolumetoatotalof10partswithsterilewater.
  4. Topreventgelation,maintaintemperatureofmixtureat2-10°C.
  5. Toformgel,warmto37°C.Forbestresultsallowapproximately40minutesforgelformation.

ProductQ&A

WecompletedastudytoshowthatDNAiscompletelydestroyedatpH2,anddemonstratedthatourcollagenproductsdonotcontainDNA.

Thecollagenisfullyhydrolyzed.TheaminoacidanalysisisdoneusingtheWatersAccQ-Tagderivatizationmethod.Duringtheacidhydrolysisstep,asparagine(N)isconvertedtoasparticacid(D)andglutamine(Q)isconvertedtoglutamicacid(E).Tryptophan(W),ifpresent,isdestroyedduringacidhydrolysis.Experimentally,onecandeterminethepicomoles(pmol)ofeachaminoacidperinjecteddetectedusingaminoacidstandards.Fortheconcentrationdetermination,thetotalnumberofpmolofeachaminoacidissummedtogetthetotalpmolofthe18aminoacidsdetected.Thetotalpmolaminoacidsisdividedbythetheoreticalnumberofaminoacidresiduesincollagenbasedonthepublishedsequence.Theresultisthepmolofcollageninjected.Theresultisthenmultipliedbythedilutionand300,000isusedasthecollagenmolecularweighttogettomg/mL.Themolecularweightofcollagenisnotwellagreedupon.

Dilutingwith1XPBS(ratherthanwateror0.01NHCl)wouldhaveaneffectforcoatingpurposes.ItwouldchangethepHofthedilutedcollagensolutionfromacidtoneutralpH.ThepHchangewilltransformthecollagenmoleculesfromamolecularformtoafibrillarform;andthenthenatureofcoatingsurfacewillbechangedfromamonomericcoatingtoafibrillarcoating.

WeusethefollowingantibodiesfromSouthernBiotech:

1.1310-02–GoatAnti-TypeICollagen-FITC

2.1310-08–GoatAnti-TypeICollagen-BIOT

3.7100-05–Streptavidin-HRP

ThemajorcollagenmolecularspeciesinourTypeIcollagenproductsaremonomers(approx.70%),buttherearedimers,trimersandafewpercentagesofoligomerstoo(approx.30%)withsomeminoramountsofcollagenfragments.Thecollagenmonomerisarodshapedmoleculewith300nminlengthand1.5nmindiameter.Thedimer,trimerandoligomerare600nm,900nmandevenlongerinlengthrespectively.Accordingtothecoatingprocedures,thecollagenmoleculesareattachedtothechargedpolystyrenesurfacerandomlybychargeoraffinityinacidconditionsduringthe1-2hrsincubationperiodat37°C,andanyunattachedmaterialsareremovedbyaspirationandrinsing.Therefore,thecoatedsurfaceisasinglelayerofcollagenmonomer,dimer,trimerandoligomermixtures.Thethicknessofthemono-molecularlayerisdependentonhowthosemoleculesareattachedonthesurface.Thecoatingdensitythicknesswouldgenerallybecharacterizedasa1moleculethicknesswhichcouldberangingfromafewnanometerstoafewhundrednanometerswiththewholesurfacebeingcoveredbycollagen.

ThenetchargeofTypeIcollagenproducts’(PureCol®,BovineCollagenandVitroCol®,HumanCollagen)moleculeisdirectlyrelatedtothepH.AtanacidicpH,theaminoacids(zwitterions)alongthecollagenmoleculearepositivelycharged,makingtheentirecollagenmoleculepositive.Attheisoelectricpoint(orzone)ofcollagen,aroundpH7-8,theaminoacidsalongthecollagenmoleculearepositivelyandnegativelycharged,makingthenetchargeofthecollagenmoleculeclosetozero.AtabasicpH,theaminoacidsalongthecollagenmoleculewerenegativelycharged,makingtheentirecollagenmoleculenegative.

Further,thenatureofthechargeofthecollagencoatingsurfacewillbedependentonthetypeofcoatingapplied.ForamonomericcollagencoatingswhenthecollagenisappliedunderanacidicpHcondition,thesurfaceispositivelycharged.IfthesurfaceisrinsedwithpHneutralbufferormediathenitwillchangethechargeofthecollagensurfacenetchargeclosetozero.Fora3Dgelcoating,thecollagenpreparedunderneutralpH;thenetchargeofthecollagensurfaceisclosetozero.

Usingrotaryshadowingtechniqueundertransmissionelectronmicroscopy,itwasfoundthatourcollagen,onaverage,consistsofapproximately80%monomers,13%dimers,trimers,andoligomerswiththeremaining7%collagenfragments.

Yes.ThecollagenmoleculeinPureCol,Nutragen,VitroCol,andallofourotherAtelocollagenproductswerepreparedfromnativecollagenmatrixbypepsintreatmentundercontrolledconditionstoremovethenon-helicalportion,telo-peptides,onlyandthehelicalportionisintact.Inthiscase,theenzymaticactivesitesforMMP(MatrixMetalloproteinase),suchasforMammalianCollagenaseMatrixMetalloproteinase8(MMP-8),onthemoleculewaspreserved.

Thesepepsintreatedcollagenproductsshouldbehaveasnativeintactcollagen.

TGFbetawouldhavebeendigestedwiththepepsinenzymaticdigestionstep.ItwasundetectablebySDSPAGEsilverstainaswell.Wedidn’tdoanyspecificmeasurementsbyELISAhoweverbutpresencesofTGFbetaisnotanticipated.

WeprimarilyusetheBiuretmethod,butwealsouseBCA,AAA,andhydroxyl-prolineassays.

-Collagensolutionsthatarefrozentendtohaveissuesforming3Dhydrogels,andwilllikelynotwork.Thesolutionsshouldstillbegoodfor2Dcoatings.

-Collagensolutionsthatareleftoutatroomtemperatureforextendedperiodsoftimemayshowsignsofdegradation,whichwillaffecttheformationof3Dhydrogels.Itislikelystillfinefor2Dcoatings.

Ourrecommendationisthis:Ifyouareusingtheproductdirectlyforapublication,wehighlysuggestbuyinganewbottleiftheoneyouhavewascompromised.

ProductReferences

ReferencesforNutragen®:

Tanic,J.,etal."AdseverinmodulatesmorphologyandinvasivefunctionofMCF7cells."BiochimicaetBiophysicaActa(BBA)-MolecularBasisofDisease(2019).

Rosell-García,Tamara,etal."DifferentialcleavageoflysyloxidasebythemetalloproteinasesBMP1andADAMTS2/14regulatescollagenbindingthroughatyrosinesulfatedomain."JournalofBIOLOGicalChemistry(2019):jbc-RA119.

Berger,AnthonyJ.DecouplingthePropertiesofthePhysicalMicroenvironment:TheDesignandApplicationofaGelatin-Methacrylate/CollagenCompositeHydrogel.Diss.TheUniversityofWisconsin-Madison,2018.

Berger,AnthonyJ.DecouplingthePropertiesofthePhysicalMicroenvironment:TheDesignandApplicationofaGelatin-Methacrylate/CollagenCompositeHydrogel.Diss.TheUniversityofWisconsin-Madison,2018.

Renkawitz,Jörg,etal."Nuclearpositioningfacilitatesamoeboidmigrationalongthepathofleastresistance."Nature568.7753(2019):546.

Szulc,DanielAndrzej,andHai‐LingMargaretCheng."One‐StepLabelingofCollagenHydrogelswithPolydopamineandManganesePorphyrinforNon‐InvasiveScaffoldTrackingonMagneticResonanceImaging."Macromolecularbioscience(2019):1800330.

Formica,FlorianA.,GoncaloBarreto,andMarcyZenobi-Wong."Cartilage-targetingdexamethasoneprodrugsincreasetheefficacyofdexamethasone."JournalofControlledRelease295(2019):118-129.

Dubois,Fatéméh,etal."AroleforRASSF1AintunnelingnanotubeformationbetweencellsthroughGEFH1/Rab11pathwaycontrol."CellCommunicationandSignaling16.1(2018):66.

Čermák,Vladimír,etal."RNA-seqofmacrophagesofamoeboidormesenchymalmigratoryphenotypeduetospecificstructureofenvironment."Scientificdata5(2018):180198.

ProductCertificateofAnalysis

Noresultfor.

ProductVideos

link to library blog - How to Make 3D Collagen Hydrogels
HowtoMake3DCollagenHydrogels

Video

link to library blog - Telo vs Atelo Collagen
TelovsAteloCollagen

Video

link to library blog - Seeding Collagen Gels with Cells
SeedingCollagenGelswithCells

Video

link to library blog - 4 Key Components for 3D Collagen Gels
4KeyComponentsfor3DCollagenGels

Video

link to library blog - Collagen Concentration vs Gel Stiffness
CollagenConcentrationvsGelStiffness

Video

link to library blog - Coating Plates with Collagen
CoatingPlateswithCollagen

Video

link to library blog - Coating a Glass Coverslip with Collagen
CoatingaGlassCoverslipwithCollagen

Video

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SafetyandDocumentation

SafetyDataSheet

CertificateofOrigin

DeclarationofMaterialSource

ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

品牌介绍
美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量,并保证产品之间一致性,方便研究客户使用。 以下为Advanced BioMatrix 3D Matrices 产品竞争优势: 1. 提供高纯度和成分确定的胞外基质; 2. 超过1000余篇文献引用PureCol产品,品质非常均一; 3. 在3D培养基领域可提供最全面的产品线; 4. 唯一可提供特异性刚性有机硅基板的公司(CytoSoft); 5. 唯一可提供可溶性丝纤蛋白的供应商(可运用于多种3D培养); 6. 如果客户首次接触3D胶原凝胶,Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。 以下产品为Advanced BioMatrix全球畅销品: 1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML 2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML 3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML 4. VitroCol 人源I型胶原蛋白 #5007-20ML 5. 弹性蛋白原 #5052-1MG 6. ECM Select Array kit Ultra-36 #5170-1EA 7. CytoSoft(刚性可变的基底,Advanced BioMatrix最新添加产品5190-7EA) 8. 人III型胶原蛋白 #5021-10MG 9. 人IV型胶原蛋白 #5022-5MG 10. Silk Fibroin溶液 #5154-20ML 11. Fibronectin #5080-5MG 12. Vitronectin #5051-0.1MG Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。